Anti-SARS-CoV-2 Neutralization Antibody Test Kit (Enzyme-linked immunosorbent assay)

  • Cat.No.:CP04012
  • Format:ELISA
  • Reading Time:About 2h
  • Sample Type:Serum and Plasma
  • Size:96T/5*96T
  • Lead Time:Order now, ship in 3 days
Filter:SARS-CoV-2

Product Introduction

It is a semi quantitative competition ELISA kit to detect the Anti-SARS-CoV-2 Neutralization Antibody in human serum or plasma. 
The micro test plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 neutralization antibody in the standard & sample diluent pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

Product Features

High-throughput screenin

Safety/stability

Time saving

Test Procedure

Determine wells for standard reference and negative controls and samples. Add 50μL each pre-treated samples and controls into the appropriate wells (It is recommended that all samples and controls be assayed in duplicate). Immediately add 50μL of HRP conjugated SARS-CoV-2 RBD fragment (HRP-RBD) working solution to each well. Cover the plate with the sealer provided in the kit. Incubate for 60 min at 37℃ .
Note:solutions should be added to the bottom of the micro test plate well, avoid touching the inside wall and causing foaming as much as possible.

Decant the solution from each well, add 350μL of wash buffer to each well. Soak for 30-60 seconds and aspirate or decant the solution from each well and pat it dry against clean absorbent paper. Repeat this wash step 3 times.
Note:a microplate washer can be used in this step and other wash steps. Make the tested strips in use immediately after the wash step. Do not allow wells to be dry.

Add 90μL of substrate reagent to each well. Cover the plate with a new sealer. Incubate for about 15 min at 37℃ . Protect the plate from light.
Note: the reaction time can be extended according to the actual color change, but not more than 30min. Preheat the microplate reader for about 15 min before OD measurement.

Add 50μL of stop solution to each well.
Note:adding the stop solution should be done in the same order as the substrate solution.

Determine the optical density (OD value) of each well at once with a micro-plate reader set to 450 nm.

Testing Results

In the range of [0.05 AU/mL, 480AU/mL], the correlation coefficient r≥0.990. Normal reference value: neutralization antibodies < 19.5AU/mL.

Kit Components & Storage

Components
Components 96T 480T
Micro test plate (Dismountable)  8 wells*12 strips, 1 plate 8 wellsx12 strips, 5 plates
Standard reference 2 vials 10 vials 
Negative control 2 vials 10 vials 
Standard & sample diluent 1 vial, 20 mL 5 vials, 20 mL
Concentated HRP conjugated RBD(HRP-RBD, 100x) 1 vial, 120μL 5 vials, 120μL
HRP conjugate diluent 1 vial, 10 mL 5 vials, 10 mL
Concentrated wash buffer(25x)  1 vial, 30 mL 5 vials, 30 mL
TMB substrate solution 1 vial, 10 mL 5 vials, 10 mL 
Stop solution 1 vial, 10 mL 5 vials, 10 mL
Plate sealer 3 pieces 15 pieces
Product description   1 copy 1 copy
Storage

1. The test kit has to be stored at 2-8℃ , do not freeze and avoid exposure to direct sunlight. The unopened is stable for at least 6 months.

2. The opened kit can be stored at 2-8℃ for 2 weeks.

About UNscience

UNscience, a wholly owned subsidiary of Elabscience, specializes in the research and development, production and sale of in-vitro diagnostic reagents. Certified with ISO 13485 and other documents, UNscience sell its products all over the world.

The company has 100,000 grade GMP purification workshop and quality management system, three major technical platforms (Colloidal Gold Immunochromatographic Platform, Fluorescence Immunochromatographic Platform, and Pathological Diagnosis Antibody Platform). UNscience has the independent research and development and production capacity of core raw materials, and has successfully developed 27 POCT immunochromatographic quantitative detection products (colloidal gold and fluorescence), mainly covering cardiovascular and cerebrovascular diseases, kidney diseases, diabetes, infectious diseases, reproductive health, health examination and other.